Gas producing Collinsella bacteria|364165 |BNCC

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Gas producing Collinsella bacteria-BNCC
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  • Collinsella aerofaciens-BNCC

Collinsella aerofaciens

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  • number：364165
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    The colonies measure 0.5-1 mm in diameter, are circular with regular margins, and are opaque. They exhibit a grayish-white pigmentation on the surface, have a flat morphology, and are smooth and moist in texture, making them easy to lift. No pigment is produced. The organisms are Gram-positive (G⁺, appearing blue-violet) and rod-shaped (bacilli). Purity: pure.
Standard strain Quantitative strain DNA extraction
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Collinsella aerofaciens
Subculture procedure ① Dissolve the frozen tube in a 37 ℃ water bath and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (each Agar plate contains approximately 200 μ L, and for anaerobic bacteria cultivation, the culture medium needs to be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Growth conditions 37 ℃; 48-72 hours; anaerobic
Storage conditions -80 ℃
Safety level 1
morphology The colonies measure 0.5-1 mm in diameter, are circular with regular margins, and are opaque. They exhibit a grayish-white pigmentation on the surface, have a flat morphology, and are smooth and moist in texture, making them easy to lift. No pigment is produced. The organisms are Gram-positive (G⁺, appearing blue-violet) and rod-shaped (bacilli). Purity: pure.
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