Citrobacter freundii|186185 |BNCC

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Citrobacter freundii

Subculture procedure	① Prepare 2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	37 ℃; 18-24h; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Colony diameter: 1–2 mm; round with entire margins; opaque; pale yellow on the obverse; convex center; smooth, glistening surface; moist consistency; easily lifted; non-pigmented; Gram-negative (red); rod-shaped; pure culture.
Sharing mode	Public welfare sharing

Citrobacter freundii

Description

BNCC No.: 186185

Biosafety level: 4

Storage conditions

Storage of freezed dried ampoule and agar slant at 2°C to 8°C.

Growth Conditions

1. Nutritious gravy agar: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 15.0g, distilled water 1.0L,pH7.0. [note] adding 5mg MnSO_4.H2O when culturing Bacillus,which is beneficial to spore production. pH7.0. 121 ℃,15min.

2. Atmosphere: aerobic

3. Temperature: 37 ℃,24h

4. Notes

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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