Clostridium sporogenes|273545 |BNCC

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Clostridium sporogenes (Metchnikoff) Bergey et al.

Culture medium	BNCC359963
Description	Trypticase Soy Blood Agar
Composition	Tryptone:15.0g, Soy Peptone:5.0g, NaCl:5.0g, Sterile Defibrinated Sheep Blood:5%, Agar:15.0g, pH:7.3±0.2(25℃)
Growth conditions	37 ℃; 24-48 hours; anaerobic;
Subculture procedure	① Dissolve the frozen tube in a water bath (fungi at 30 ℃, bacteria at 37 ℃) and shake rapidly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (each Agar plate contains approximately 200 μ L, and for anaerobic bacteria cultivation, the culture medium needs to be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Storage conditions	-80 ℃
Safety level	1
morphology	The diameter of the colony is 2-4m, with a central protrusion, gray white color, pseudo root like edges, semi transparent, and a dull surface. It is G+(blue purple), rod-shaped, and has oval shaped spores. Purity: pure
Sharing mode	Public welfare sharing