BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC360928 |
| Description | Buffered Charcoal Yeast Extract Agar |
| Composition | Yeast Extract Powder:10.0g, Activated Charcoal:2.0g, Agar:13.0g, α-Ketoglutarate Monopotassium Salt(C5H5KO5):1.0g, ACES Buffer:10.0g, Potassium Hydroxide(KOH):2.8g, Ferric Pyrophosphate(Fe4(P2O7)3):0.25g |
| Growth conditions | 37 ℃; 48-72h; 5% CO2 |
| Subculture procedure | ① Prepare 1-2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 2 |
| morphology | Colony diameter: 1–4 mm; round with irregular margins; opaque; grayish-white on the obverse; slightly convex center; rough surface; easily lifted; Gram-negative (red); rod-shaped; pure culture. |
| Sharing mode | Public welfare sharing |
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