BeNa Culture Collection
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| Culture medium | BNCC352251 |
| Description | YM agar medium |
| Composition | Yeast Extract Powder:3.0g, Malt Extract:3.0g, Glucose:10.0g, Casein Peptone:5.0g, Agar:20.0g, pH:6.2±0.2(25℃) |
| Growth conditions | 30°C; 24-48h; aerobic |
| Subculture procedure | ① Prepare 1-2 plates as above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then immediately add sterile water to rupture; break open with forceps; ③ Pipette 0.5mL sterile water into lyophilized tube, dissolve powder completely, then dispense 200μL/well onto plates, spreading evenly; ④ Incubate plates under specified conditions until colonies emerge. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 1-2 mm; circular shape with neat margins; opaque, creamy-white surface; central elevation; smooth texture; easily liftable; Gram-positive (blue-violet); coccobacillus |
| Sharing mode | Public welfare sharing |
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