Streptomyces albus|153223 |BNCC

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Streptomyces albulus

Subculture procedure	① Prepare 1-2 plates as above; ② Disinfect the Agar plate surface and open it in a safety cabinet; ③ Pipette 1-3 ml sterile water onto the Agar plate; use a sterile spreader to scrape the bacterial layer back and forth across the Agar plate surface to create a bacterial suspension; ④ Pipette the suspension with a Pasteur pipette and dispense onto fresh plates, spreading evenly; ⑤ Incubate plates under the specified conditions.
Growth conditions	28°C, 48-72h, aerobic
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1-2 mm; irregular shape with jagged edges; opaque, pale yellow surface; flat morphology; rough texture; dry consistency; Gram-positive (blue-violet); bacilli
Sharing mode	Public welfare sharing

Streptomyces albicolor

Storage conditions : 2~8 ℃

No. : 153223

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 1, safety cabinet operation

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 28 ℃, aerobic, ISP-2,48-72h. ISP-2: yeast powder 4.0g, malt powder 10.0g, glucose 4.0g, agar 20.0g,pH 7.2 ±0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1)Prepare 1-2 of above mentioned plates;

(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) The above plate is cultured under the above-mentioned culture conditions, and the bacteria can be used after growth.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 72hours strain layer become obvious;colony is typical on the streaked plate
colony morphology: (above)	Size: 1-2mm Shape: Irregular Edge: Untidy Transparency: Opaque Color: Light Yellow Bulge: Flat Surface: Rough Texture: Dry
Conclusion:	good viability, no abnormal colony morphology, qualified

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