Purple Monascus|189221 |BNCC

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Monascus purpureus

Subculture procedure	① Prepare 1-2 plates as mentioned above; ② After disinfecting the inclined surface of the test tube, open it in the safety cabinet; ③ Cut small square blocks of 0.5 × 0.5cm2 using a sterile inoculation hoe and inoculation shovel (see attached page); ④ Place the small square block flat on the center of the Agar plate and onto the surface of the agar; ⑤ Cultivate the flat Agar plate under the above cultivation conditions, and the strain can be used once it grows.
Growth conditions	28 ℃; 3-5 days; aerobic
Storage conditions	-80℃
Safety level	1
morphology	Small filamentous fungi produce distinct, purplish-red, velvety colonies on comprehensive PDA medium, with dense, low-lying mycelium.
Sharing mode	Public welfare sharing

1. Description

1. Name: Monascus purpureus

2. BNCC No.: 189221

3. Biosafety level: 4

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C.

3. Growth Conditions:

1. Wort agar medium: 130g malt paste powder, 15g agar, 0.1g chloramphenicol, 1L distilled water, pH6.0±0.2,121 ℃,15min.

2. Atmosphere: aerobic

3. Temperature: 25-28 ℃

4. Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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