Fusarium|189975 |BNCC

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Fusarium proliferatum

Subculture procedure	① Thaw Frozen vial in water bath (30°C for fungi), dissolve by vigorous shaking; ② Disinfect outer surface of vial with alcohol swab, then transfer to safety cabinet for handling; ③ Unscrew cap, aspirate entire thawed solution, and inoculate 1-2 agar plates; ④ Spread evenly and incubate under specified conditions.
Growth conditions	28°C; 5-7 days; aerobic
Storage conditions	-80°C
Safety level	1
morphology	Small filamentous fungus. Colony formation is distinct on PDA medium. Mycelium is white, dense, and vigorous, spreading concentrically toward the Agar plate edge. Does not produce spores. The underside of the Agar plate appears orange-yellow, indicating good viability.
Sharing mode	Public welfare sharing

Fusarium proliferatum

No.: 189975

Storage conditions: 2~8 ℃

Product format: agar slant in 14mm test tube

Validity period: 30 days

Biosafety level: 1,handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28 ℃, aerobic, integrated PDA,5-7 days. Comprehensive PDA:20% potato juice 1L, glucose 20g ,KH₂PO₄ 3g, MgSO₄.7H₂O 1.5g, thiamine trace, agar 15g,pH natural.

Recovery steps:

(1)Prepare 1-2 pieces of PDA plates;

(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3) Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2;

(4) Lay flat the small pieces to the center of the agar plate;

(5)Put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the cell recovery are reported as follows:

item	test result
Viability:	good viability,in 7 days plate colony is obvious
colony morphology:	small filamentous fungi, with obvious colonies, white hyphae, dense and vigorous on comprehensive PDA medium, Spread and grow to concentric circles on the edge of the plate without spores. The back of the plate is orange and has good activity.
conclusion:	good viability, no abnormal colony morphology, qualified

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