Slow growing rhizobia|232076 |BNCC

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Bradyrhizobium elkanii

Subculture procedure	① Dissolve the frozen tube in a 30 ℃ water bath and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the cap of the tube, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (approximately 200 μ L per Agar plate); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Growth conditions	30 ℃; 3-5 days; aerobic
Storage conditions	-80 ℃
Safety level	1
morphology	Size: 1-2mm Shape: Circular Edge: Irregular Transparency: Opaque Color: Pink Rise: Middle Rise Surface: Bright and Smooth Texture: Wet and easy to pick up, G - (red), Bacillus, Purity: Pure
Sharing mode	Public welfare sharing

Bradyrhizobium elkanii

Storage conditions : 2~8 ℃

No. 232076

Product format: freeze dried, 200ul

Validity : 6 years

Biosafety level: 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :30 ℃, aerobic, rhizobia YMA medium, 3-5 days. Rhizobium YMA medium: KH2PO4 0.25g,K2HPO4 0.25g,MgSO4 ·7 H2O 0.2g,NaCl 0.1g, yeast extract 0.8g, mannitol 10.0g, agar 18.0g, distilled water 1.0 L,pH 7.2,121 ℃,15min.

Recovery steps:
(1) Prepare 1-2 of above mentioned plates;
(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;
(4) Put the plates under the above culture conditions for cultivation for 3-5days.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 5 days strain layer become obvious, colony is typical
colony morphology: (above)	Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opaque color: pink bulge: middle bulge surface: bright and smooth texture: moist and easy to stir
Conclusion:	good viability, no abnormal colony morphology, qualified

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