BeNa Culture Collection
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| Culture medium | BNCC352239 |
| Description | Nutrient Agar |
| Composition | Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃) |
| Growth conditions | 30°C; 18-24h; aerobic |
| Subculture procedure | ① Prepare 1-2 fresh plates as described above; ② Open in a biosafety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to break it open; use forceps to crush it; ③ Pipette 0.5mL sterile water into the freeze-dried vial, dissolve completely, and mix thoroughly; ④ Pipette 0.2mL of the bacterial suspension onto the agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate under the specified conditions; the strain is ready for use once colonies appear. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 0.5-1mm; circular with irregular margins; opaque; white upper surface; centrally raised; rough texture; moist consistency; easily lifted; G+ (blue-violet); bacillus; purity: pure |
| Sharing mode | Public welfare sharing |
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