Bacillus subtilis subsp. subtilis|293727 |BNCC

Essential Information Related Products

Bacillus spizizenii (Nakamura et al.) Dunlap et al.

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	30°C; 18-24h; aerobic
Subculture procedure	① Prepare 1-2 fresh plates as described above; ② Open in a biosafety cabinet, cauterize the top with a酒精灯, then quickly add sterile water to break it open; use forceps to crush it; ③ Pipette 0.5mL sterile water into the freeze-dried vial, dissolve completely, and mix thoroughly; ④ Pipette 0.2mL of the bacterial suspension onto the agar plate, spread evenly; repeat twice to obtain two plates; ⑤ Incubate under the specified conditions; the strain is ready for use once colonies appear.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 0.5-1mm; circular with irregular margins; opaque; white upper surface; centrally raised; rough texture; moist consistency; easily lifted; G+ (blue-violet); bacillus; purity: pure
Sharing mode	Public welfare sharing