Long branch Trichoderma|336351 |BNCC

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Trichoderma longibrachiatum

Subculture procedure	① Prepare 1-2 plates as mentioned above; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each Agar plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	28 ℃; 5-7 days; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	Spread and grow, produce a large number of spores, produce yellow spores, irregular edges, yellow front, flat shape, rough surface, wrinkled surface, dry texture, purity: pure
Sharing mode	Public welfare sharing

Trichoderma viride Persoon

BNCC No.:336351

Biosafety level : 4

Storage conditions: Storage of freezed dried ampoule and agar slant at 2°C to 8°C

Growth Conditions

1.Comprehensive potato culture medium: 20% potato juice 1L, glucose 20g, KH2PO4 3g ,MgSO4.7H2O 1.5g ,Vitanib B1 (thiamine) trace, agar 15g, pH 6

2. Atmosphere:erobic

3. Temperature: 25-28℃

4.Notes:

1.Normal culturing time, 1-2days for bacterial, 3 days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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