Purple red Aspergillus|336565 |BNCC

Essential Information Certificate Related Products

Monascus purpureus Went

Subculture procedure	① Dissolve the frozen tube in a water bath (fungi at 30 ℃) and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates; ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Growth conditions	28 ℃; 7-10 days; aerobic;
Storage conditions	-80 ℃
Safety level	1
morphology	Spread growth, filamentous, irregular edges, reddish brown front, rough surface, difficult to pick/cut, purity: pure
Sharing mode	Public welfare sharing

Monascus purpureus Went

Storage conditions: 2~8 ℃

No. 336565

Product format: freeze dried, 200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Recovery/subculture: 28 ℃, aerobic, 10d, wort agar: malt extract 20.0g, glucose 20.0g, peptone 1.0g, agar 20.0g, distilled water 1.0L,pH natural. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1)Prepare 2 wort agar plates;

(2) Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
Viability:	good viability, in 10 days plate colony is obvious
colony morphology:	Small filamentous fungus, growing well on wort agar medium, with purple-red colonies, raised colonies in the middle, with folds and radiation stripes.
Conclusion:	good viability, no abnormal colony morphology, completely consistent with the above figure, qualified

Download certificate