Lactobacillus delbrueckii subsp. lactis|337402 |BNCC

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Lactobacillus delbrueckii subsp. lactis

Culture medium	BNCC352238
Description	MRS agar medium
Composition	Peptone:10.0g, Beef Extract Powder:8.0g, Yeast Powder:4.0g, Glucose:20.0g, MgSO₄:0.2g, CH₃COONa:5.0g, C₆H₈O₇.(NH₄)₂:2.0g, K₂HPO₄:2.0g, MnSO₄:0.04g, Tween 80:1.0g, Agar:14.0g, pH:5.7±0.2(25℃)
Growth conditions	37°C; 24-48h; anaerobic
Subculture procedure	① Prepare 1-2 plates as above and sterile water (pre-placed in anaerobic environment for 24h to remove oxygen); ② Open in a safety cabinet, cauterize the top with a spirit lamp, then quickly add sterile water to rupture it; break it into pieces with forceps; ③ Pipette 0.5mL sterile water into the freeze-dried vial, dissolve the powder thoroughly, then dispense 200μL/well onto plates and spread evenly; ④ Incubate plates under specified conditions until colonies emerge.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 1-2 mm; circular, with neat, opaque edges and a raised center. Surface: grayish-white, smooth, glossy, moist texture, easily liftable. Gram-positive (blue-violet), bacilli. Purity: Pure
Sharing mode	Public welfare sharing