BeNa Culture Collection
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| Culture medium | BNCC360853 |
| Description | Arginine Thymine Yeast extract Pyruvate Agar |
| Composition | NH4Cl:1.0g, KH2PO4:0.5g, MgCl2:0.5g, CaCl2:0.1g, NaCl:1.0g, CH3COONa:2.0g, Sodium Succinate(C4H4Na2O4):1.0g, Yeast Extract Powder:0.5g, Peptone:0.5g, FeCl2:1.8mg, CoCl2:0.25mg, NiCl2:0.01mg, CuCl2:0.01mg, MnCl2:0.07mg, ZnCl2:0.1mg, H3BO3:0.5mg, Na2SeO3:0.01mg, Na2MoO4:0.01g, Biotin(C10H16N2O3S):0.01mg, Nicotinic Acid(C6H5NO2):0.035mg, Calcium Pantothenate(C9H16NO5Ca):0.01mg, Thiamine Hydrochloride(C12H17ClN4OS.HCl):0.03mg, Vitamin B12(C63H88CoN14O14P):0.005mg, Pyridoxamine Hydrochloride(C8H12N2O2.HCl):0.01mg, p-Aminobenzoic Acid(C7H7NO2):0.02mg, Agar:13.0g, pH:6.8(25℃) |
| Growth conditions | 30°C, 5-7 days, anaerobic light |
| Subculture procedure | ① Thaw Frozen vials in a 30°C water bath, shaking vigorously to dissolve; ② Disinfect outer surface of Frozen vials with alcohol swabs, then transfer to safety cabinet for handling; ③ Unscrew the cap, aspirate the entire thawed contents into a test tube; ④ Incubate under the specified conditions. |
| Storage conditions | -80°C |
| Safety level | 1 |
| morphology | Colony diameter 1-2 mm, circular, with regular margins; opaque, red on top, centrally convex, smooth surface, moist texture, easily liftable; G- (red), bacillus; Purity: Pure |
| Sharing mode | Public welfare sharing |
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