Botrytis cinerea|338228 |BNCC

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Botrytis cinerea

Subculture procedure	① Prepare 1-2 plates as mentioned above; ② After disinfecting the surface of the tablet, open it in the safety cabinet; ③ Take 1-3ml of sterile water and pour it into the Agar plate. Use a sterile coating rod to scrape the bacterial coating back and forth on the surface of the Agar plate to make a bacterial suspension; ④ Suck the bacterial suspension with a pipette, pour it onto a fresh Agar plate, and apply evenly; ⑤ Place the agar plate under the above cultivation conditions for cultivation.
Growth conditions	28 ℃; 5-7 days; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, spreading and growing, filamentous, with irregular edges, opaque, gray white on the front and gray white on the back, protruding in the middle, rough surface, dry texture, purity: pure
Sharing mode	Public welfare sharing

Botrytis cinerea

storage conditions: 2~8 ℃

number: 338228

product format: agar slant in 14mm test tube

Validity: growing culture, in 30 dyas

Biosafety level : 1, ultra-clean desk or safety cabinet operation

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The agar slant can be used directly. In principle, it can be used many times without contamination within the validity period, but viability will gradually decrease with time. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

culture conditions: 25-28 ℃, strict aerobic, comprehensive PDA,10-14 days. Comprehensive PDA agar: potato boil 1.0L, glucose 20.0g,KH₂PO₄ 3.0g, MgSO₄·7H₂O 1.5g, little vitamin B1, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.

Recovery steps:

(1)Prepare 1-2 pieces of PDA plates;

(2)sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3)cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2;

(4)lay flat the small pieces to the center of the agar plate;

(5)put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
vaibility	good vaibility. in10-14 days colony is obvious on the plate
colony morphology	small filamentous fungi have obvious colonies on comprehensive PDA medium, white hyphae, dense and vigorous, spreading to concentric circles at the edge of the plate and not producing spores
Conclusion	good viability, no abnormal colony morphology, qualified

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