Orange green wood fungi|342638 |BNCC

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Trichoderma citrinoviride Bissett

Subculture procedure	① Prepare 1-2 tablets mentioned above; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	28 ℃; 3-5 days; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	Small filamentous fungi have obvious colonies on comprehensive PDA medium, with initially gray white hyphae and sparse hyphae, producing yellow spores later and green spores later on
Sharing mode	Public welfare sharing

Trichoderma citrinoviride Bissett

Storage conditions : 2~8 ℃

No. : 342638

Product format: freeze dried, 200ul

Validity period: 6 years

Biosafety level : 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Growth conditions :28 ℃, aerobic, comprehensive PDA,3-5 days, comprehensive PDA: potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4·7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.

Recovery steps:
(1)Prepare 1-2 of above mentioned plates;
(2)Open the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3)Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;
(4)Put the plates under the above culture conditions for cultivation for 3-5 days.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability, in 3-5 days strain layer is obvious
colony morphology: (above)	small filamentous fungi have obvious colonies on comprehensive PDA medium, white initial hyphae, dense and vigorous, and yellow spores.
conclusion	good viability, no abnormal colony morphology, qualified

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