Salmonella enterica subsp. enterotoxum infant serotype|353759 |BNCC

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Salmonella enterica subsp. enterica serovar Infantis

Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each Agar plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	37 ℃; 18-24h; aerobic;
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, gray white front, raised middle, smooth surface, bright surface, moist texture, easy to pick
Sharing mode	Public welfare sharing

Salmonella enteritidis

No. : 353759

Product format: tablets in penicillin bottle

Storage conditions : -20 ℃

Biosafety level : 2

Validity period: 5 years

Use : pharmacopoeia, national standard and other control quality control strains & nbsp;

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. The bacterial tablets shall be used up once and shall not be retained once the penicillin bottled is opened. Keep it at -20℃ for storage if it is not used. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Medium : nutrition gravy medium beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH7.0. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1)Prepare a sterile test tube of 5~10ml liquid medium and 2 pieces of 90mm agar plates;
(2)Sterilizing of the ampoule, under aseptic conditions, remove the cap and take out the inner vial. lift the cotton plug, rehydrate the pellets by drawing 0.5ml of liquid culture medium into the vial.
(3)Fully dissolved, transfer the solution to the liquid test tube, mix evenly;
(4)Inoculate a plate with the solution, distribute it well in 200ul/ plate; the plates are not allowed to seal by wrapping.
(5)Put all the plates and test tubes under the above culture conditions for cultivation, The culture solution is obviously turbid after 18-24 hours, and this indicates the bacterial grow well.
(6)If the strain does not grow, it should be continued to be cultured for 72h, and then used according to the test requirements.

Growth conditions temperature: 37 ℃ Gas environment: aerobic Time: 18-24h

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