Ganoderma lucidum|360081 |BNCC

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Ganoderma lucidum

Subculture procedure	① Prepare one 90mm solid Agar plate, one inoculation shovel and one inoculation hoe; ② Inoculation shovel, inoculation hoe, flame burn, cool for later use; ③ After disinfecting the outer wall of the inclined surface, open it in a sterile environment, first use an inoculation hoe, and then use an inoculation shovel to cut into pieces; ④ Inoculate the bacterial block with a spatula, place it on the surface of a agar plate, and cover the Agar plate; ⑤ Place the flat Agar plate in the fungi incubator and cultivate it according to the above conditions. Do not seal it with a sealing film. The Agar plate can be used when it is almost full.
Growth conditions	28 ℃; 7-14 days; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Small filamentous fungus, spreading and growing, grows well on comprehensive PDA medium, with white front and yellow back of the medium
Sharing mode	Public welfare sharing

Ganoderma lucidum

Storage conditions : 2~8 ℃

No. : 360081

Product format :freeze dried, 200ul

Validity : 6 years

Biosafety level : 1, handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions： 28 ℃, aerobic, integrated PDA,5-7 days.

Comprehensive PDA: potato boiling solution 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4·7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L.

Recovery steps:

(1)Prepare 1-2 of above mentioned plates;

(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Put the plates under the above culture conditions for cultivation for 24-48 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 5days strain layer is obvious
colony morphology:	small filamentous fungi with obvious colonies on comprehensive PDA medium, white, The hyphae are dense and vigorous, and gray spores are produced later, spreading and growing to the edge of the plate, and the back of the medium is black
Conclusion:	good viability, no abnormal colony morphology, qualified

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