Clostridium difficile|362602 |BNCC

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Clostridium novyi

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37 ℃; 24-48 hours; anaerobic
Subculture procedure	① Thaw the cryovial in a 37 °C water bath and shake vigorously to dissolve the contents.② Wipe the outer surface of the cryovial with an alcohol swab for disinfection, then transfer it to a biosafety cabinet for further operations.③ Unscrew the cap, aspirate the entire dissolved solution, and inoculate it onto 1–2 agar plates (about 200 μL per plate; if culturing anaerobic bacteria, pre‑condition the medium in an anaerobic environment for 24 h to remove oxygen).④ Spread evenly and then transfer the plates to the specified culture conditions for incubation.
Storage conditions	-80℃
Safety level	2
morphology	The colony diameter is 1-2mm, circular, with irregular edges, opaque, gray white on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing