BeNa Culture Collection
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| Culture medium | BNCC352241 |
| Description | Columbia blood agar plate |
| Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃) |
| Growth conditions | 37°C; 3-7 days; anaerobic |
| Subculture procedure | ① Thaw Frozen vials in a 37°C water bath, shaking vigorously to dissolve; ② Disinfect outer surface of Frozen vials with alcohol swabs, then transfer to biosafety cabinet for handling; ③ Unscrew the cap, aspirate the entire thawed contents, and Agar plate onto 1-2 agar plates (approx. 200μL per Agar plate; for anaerobic cultures, pre-deoxygenate the medium in an anaerobic environment for 24 hours); ④ Spread evenly and incubate under the specified conditions. |
| Storage conditions | -80°C |
| Safety level | 2 |
| morphology | Colony diameter: 0.5–1 mm; circular, irregular margins; opaque, grayish-white upper surface with central elevation; smooth surface, easily liftable; Gram-negative (red); bacillus; purity: pure |
| Sharing mode | Public welfare sharing |
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