BeNa Culture Collection
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| Culture medium | BNCC352241 |
| Description | Columbia blood agar plate |
| Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃) |
| Growth conditions | 37°C; 48-72h; anaerobic |
| Subculture procedure | ① Thaw Frozen vial in water bath (37°C bacteria), dissolve by rapid shaking; ② Disinfect outer surface of vial with alcohol swab, then transfer to biosafety cabinet for handling; ③ Unscrew cap, aspirate entire thawed solution, and inoculate 1-2 agar plates (approx. 200μL per Agar plate; for anaerobic cultures, pre-deoxygenate medium in anaerobic conditions for 24h); ④ After uniform spreading, incubate under the aforementioned conditions. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 0.5–1 mm; circular, with neat margins; opaque, grayish-white upper surface; flat morphology; dull surface; moist texture; easily liftable; Gram-negative (red); bacillus; purity: pure |
| Sharing mode | Public welfare sharing |
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