A4 Agrobacterium tumefaciens|384628 |BNCC

Essential Information Related Products

Agrobacterium rhizogenes strain A4

Culture medium	BNCC361023
Description	LB Medium with 50μg/mL Kanamycin
Composition	Yeast Extract:5.0g, Peptone:10.0g, NaCl:10.0g, pH:7.0(25℃)
Growth conditions	28 ℃; 3-4 days; aerobic
Subculture procedure	① Dissolve the frozen tube in a 30 ℃ water bath and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the cap of the tube, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (approximately 200 μ L per Agar plate); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Storage conditions	-80 ℃
Safety level	1
morphology	The colony diameter is 0.5-1mm, circular, with neat edges, opaque, yellow on the front, light in color, raised in the middle, smooth on the surface, moist texture, easy to pick up, G - (red), rod-shaped
Sharing mode	Public welfare sharing