Lactobacillus genus|391570 |BNCC

Essential Information Related Products

Lactobacillus sp.

Culture medium	BNCC393096
Description	TYG-Acetate Agar Plate (Ready-to-use)
Composition	Tryptone:10.0g, Beef Infusion Powder:10.0g, Yeast Extract Powder:5.0g, Malt Extract Powder:150.0g, Glucose:20.0g, Ammonium Citrate Dibasic(C₆H₈O₇.(NH₄)₂):2.0g, CH₃COONa:5.0g, K₂HPO₄:2.0g, MnSO₄:0.19g, MgSO₄.7H₂O:0.58g, Tween 80:1.0mL, C₃H₇NO₂S.HCl:1.0g, Acetic Acid:20.0mL, Agar:18.0g
Growth conditions	37°C; 7-14 days; anaerobic
Subculture procedure	① Prepare 1-2 fresh plates as described above (pre-deoxygenated in anaerobic conditions for 24 hours); ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to break it. Use forceps to crush it; ③ Pipette 0.5 mL of liquid medium or sterile water (pre-deoxygenated in anaerobic conditions for 24 hours) into the freeze-dried vial. Dissolve completely and mix thoroughly; ④ Pipette 0.2 mL of the bacterial suspension into the Agar plate and spread evenly. Repeat twice to obtain two plates; ⑤ Incubate under the specified conditions. The culture is ready for use once the strain grows.
Storage conditions	2-8°C
Safety level	1
morphology	Colony diameter: 0.5–1 mm; circular, with neat edges; opaque, grayish-white on top; centrally convex; smooth surface; glossy appearance; viscous texture; moist consistency; Gram-positive (blue-violet); bacillus; Purity: Pure
Sharing mode	Public welfare sharing