BeNa Culture Collection
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| Culture medium | BNCC393096 |
| Description | TYG-Acetate Agar Plate (Ready-to-use) |
| Composition | Tryptone:10.0g, Beef Infusion Powder:10.0g, Yeast Extract Powder:5.0g, Malt Extract Powder:150.0g, Glucose:20.0g, Ammonium Citrate Dibasic(C6H8O7.(NH4)2):2.0g, CH3COONa:5.0g, K2HPO4:2.0g, MnSO4:0.19g, MgSO4.7H2O:0.58g, Tween 80:1.0mL, C3H7NO2S.HCl:1.0g, Acetic Acid:20.0mL, Agar:18.0g |
| Growth conditions | 37°C; 7-14 days; anaerobic |
| Subculture procedure | ① Prepare 1-2 fresh plates as described above (pre-deoxygenated in anaerobic conditions for 24 hours); ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to break it. Use forceps to crush it; ③ Pipette 0.5 mL of liquid medium or sterile water (pre-deoxygenated in anaerobic conditions for 24 hours) into the freeze-dried vial. Dissolve completely and mix thoroughly; ④ Pipette 0.2 mL of the bacterial suspension into the Agar plate and spread evenly. Repeat twice to obtain two plates; ⑤ Incubate under the specified conditions. The culture is ready for use once the strain grows. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 0.5–1 mm; circular, with neat edges; opaque, grayish-white on top; centrally convex; smooth surface; glossy appearance; viscous texture; moist consistency; Gram-positive (blue-violet); bacillus; Purity: Pure |
| Sharing mode | Public welfare sharing |
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