Lactobacillus acidophilus|193194 |BNCC

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Lactobacillus acidophilus-BNCC
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  • Lactobacillus acidophilus-BNCC

Lactobacillus acidophilus

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  • number：193194
  • Form：
    The colonies measure 1-2 mm in diameter, are circular with entire margins, and opaque. The colony surface is grayish-white, while the reverse side is yellow. They are raised in the center, exhibit a smooth surface and moist texture, and do not produce pigment. The cells are Gram-positive (appearing blue-violet) and rod-shaped (bacilli). The culture is pure.
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Lactobacillus acidophilus
Subculture procedure Prepare 1-2 fresh agar plates with typical bacterial colonies; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water into the freeze-drying tube, dissolve it thoroughly, and mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows.
Growth conditions 37 ℃; 24-48 hours; anaerobic
Storage conditions 2-8 ℃
Safety level 1
morphology The colonies measure 1-2 mm in diameter, are circular with entire margins, and opaque. The colony surface is grayish-white, while the reverse side is yellow. They are raised in the center, exhibit a smooth surface and moist texture, and do not produce pigment. The cells are Gram-positive (appearing blue-violet) and rod-shaped (bacilli). The culture is pure.
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Lactobacillus acidophilus

 

1.Description 

BNCC No.：193194

Biosafety level：1

2.Storage conditions：

Storage of freezed dried ampoule and agar slant at 2°C to 8°

3.Growth Conditions 

1, MRS medium: cheese peptone 10.0g, beef powder 8.0g, yeast powder 4.0g, glucose 20.0g, magnesium sulfate 0.2g, sodium acetate 5.0g, triammonium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.05g, Tween 80 1.0g, distilled water 1000mL. pH6.2 ±0.2. 121 ℃,15min.

2. Atmosphere： anaerobic

3. Temperature：37℃

4.Notes：

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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