Bacillus subtilis|195271 |BNCC

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Bacillus mucilaginosus

Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	30 ℃, 24-48 hours, aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony exhibits a diameter of 1–2 mm, with a circular shape and an irregular margin. It appears transparent, featuring a raised center, a smooth surface, and a moist texture. The colony is easily picked up, stains Gram‑positive (blue‑violet), and consists of rod‑shaped cells (bacilli).
Sharing mode	Public welfare sharing

Bacillus mucilaginosus

BNCC No.：195271

Biosafety level：4

2.Storage conditions

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1.Silicate bacteria culture medium: sucrose 5.0g, disodium hydrogen phosphate 2.0g, magnesium sulfate 0.5g, ferric chloride 0.005g, calcium carbonate 0.1g, bauxite 0.5g,pH7.1±0.2. 121 ℃,15min.

2. Atmosphere：aerobic

3. Temperature：25 ℃

4.Notes

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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