BeNa Culture Collection
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| Culture medium | BNCC360922 |
| Description | Nitrogen-Free Medium |
| Composition | Mannitol:10.0g, KH2PO4:0.2g, MgSO4.7H2O:0.2g, NaCl:0.2g, CaSO4.2H2O:0.2g, CaCO3:5.0g, Agar:15.0g, pH:7.0-7.2(25℃) |
| Growth conditions | 30 ℃, 24-48 hours, aerobic |
| Subculture procedure | ① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony exhibits a diameter of 1–2 mm, with a circular shape and an irregular margin. It appears transparent, featuring a raised center, a smooth surface, and a moist texture. The colony is easily picked up, stains Gram‑positive (blue‑violet), and consists of rod‑shaped cells (bacilli). |
| Sharing mode | Public welfare sharing |
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