Trichoderma spinosum|227595 |BNCC

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Trichoderma asperellum

Subculture procedure	① Prepare 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Suck 0.5mL sterile water into a freeze-drying tube, dissolve and mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all the plates under the above cultivation conditions, and once the strains grow, they can be used.
Growth conditions	28 ℃; 5-7 days; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	Spread and grow vigorously on PDA, with white hyphae on the front in the early stage, golden yellow to green spores on the later stage, and yellow on the back
Sharing mode	Public welfare sharing

1.Description

1. Name：Trichoderma asperellum

2. BNCC No.：227595

3. Biosafety level: 4

2. Storage conditions

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth conditions

1, PDA medium: potato extract 1.0 L, glucose 20.0g, agar 15.0g, natural pH.

2. Atmosphere：aerobic

3. Temperature：25-28 ℃

4.Notes：

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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