Fusarium|227992 |BNCC

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Fusarium sp.

Subculture procedure	① Prepare 1-2 PDA plates.② After surface sterilization of the test tube Agar slant, open it inside a biosafety cabinet.③ Using a sterile inoculation hoe and spatula, cut the culture into small square pieces measuring 0.5 × 0.5 cm² (see the attached page for details).④ Place the small square piece flat onto the center of the Agar plate, on the surface of the agar.⑤ Incubate the Agar plate upright under the aforementioned culture conditions until microbial growth is observed, after which the culture is ready for use.
Growth conditions	28 ℃, 5-7 days, aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Small filamentous fungi, forming distinct colonies on PDA medium. The mycelium is purplish-red, dense, and vigorous, exhibiting spreading growth toward the edge of the Agar plate. The reverse side of the medium appears brownish-yellow.
Sharing mode	Public welfare sharing

1.Description

1. Name：Fusarium, oxysporum f. sp. cucumerinum

2. BNCC No.：227992

3. Biosafety level：4

2.Storage conditions：

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1, PDA medium: potato extract 1.0 L, glucose 20.0g, agar 15.0g, natural pH.

2. Atmosphere：aerobic

3. Temperature：28 ℃

4.Notes：

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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