Aspergillus flavus|261205 |BNCC

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Aspergillus flavus Link

Subculture procedure	① Prepare 1-2 plates as mentioned above; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Cultivate the Agar plate under the above cultivation conditions for 5-7 days.
Growth conditions	28 ℃, 5-7 days, aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Spread and grow, initially white hyphae, later produce yellow spores, and the back of the culture medium is light yellow
Sharing mode	Public welfare sharing

Aspergillus flavus Link

Storage conditions: 2~8 ℃

No. : 261205

Product format: freeze dried, 200ul

Validity : 6 years

Biosafety level: 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The tube bevel can be used directly. In principle, it can be used many times without stain during the storage period, but the vitality will gradually decrease with time. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :28 ℃, aerobic, PDA,5-7 days. PDA: potato boiling solution 1.0L, glucose 20.0g, agar 15.0g (without liquid medium), natural pH. Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L.

Recovery steps:
(1) Prepare 1-2 of above mentioned plates;
(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;
(4) Put the plates under the above culture conditions for cultivation for 3-5 days.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability,in 7 days, strain layer is obvious
colony morphology:	Small filamentous fungi with obvious colonies on PDA medium, Early white hyphae, late yellow spores, pale yellow on the back of the medium
Conclusion:	good viability, no abnormal colony morphology, qualified ;

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