Fusobacterium nucleatum polymorphic subspecies|311817 |BNCC

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Fusobacterium nucleatum subsp. polymorphum (Knorr) Dzink et al.

Subculture procedure	① Prepare 1 tube of liquid culture medium with a diameter of 18mm, approximately 10mL (placed in an anaerobic environment for 24 hours of deoxygenation in advance); ② Disinfect the surface of the ampoule tube, open it in a safe cabinet, burn the top with an alcohol lamp, quickly add sterile water to the burning area to break it, and then use tweezers to knock it open; ③ Take about 0.5mL of liquid culture medium and transfer it into a freeze-drying tube. After fully dissolving, take it back into the liquid culture medium in the test tube and mix well; ④ Place the liquid test tube under the specified "culture conditions" mentioned above for cultivation. If the bacterial liquid is turbid or there is a large amount of bacterial growth at the bottom, it can be used.
Growth conditions	37 ℃; 4-5 days; anaerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	Fusobacterium nucleatum polymorphic subspecies, Gram negative non spore forming anaerobic bacterium, slender in shape with spindle like tips at both ends, turbid in thioglycolate liquid culture medium, liquid culture is recommended
Sharing mode	Public welfare sharing

Fusobacterium nucleatum subsp.polymorphum

No. 311817

Product format: freeze dried,200ul

Storage conditions: 2~8 ℃

Validity period: Freeze-dried tube for 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Recovery/subculture:37 ℃, anaerobic, Columbia blood plate (finished product).

Recovery steps:

(1) Prepare 1-2 of above mentioned plates;

(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Put the plates under the above culture conditions for cultivation for 24-48 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability，in 72 h, Colombian blood plate colony is obvious
colony morphology: (above)	size: small color: off-white shape: round edge: neat edge wet and dry: wet and smooth: smooth transparency: opaque uplift: uplift
Conclusion	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

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