Budding short stem fungi|335956 |BNCC

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Aureobasidium pullulans (de Bary) Arnaud

Subculture procedure	① Prepare 1-2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	28 ℃; 5-7 days; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	A filamentous fungus, forming distinct colonies on PDA medium. The colonies initially appear as white, mucoid growths from which fine hyphae emerge. Subsequently, the colonies transition to a dark brown coloration, and mature colonies present as black.
Sharing mode	Public welfare sharing

1. Description

1. Name: Aureobasidium pullulans (de Bary) Arnaud

2. BNCC No.: 335956

3. Biosafety level: 4

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1. comprehensive PDA:20% potato liquid 1.0L, glucose 20.0g, potassium dihydrogen phosphate 3.0g, magnesium sulfate heptahydrate 1.5g, vitamin B1 8mg, agar 20.0g,pH natural. 121 ℃,15min. 20% potato liquid configuration: take 200g of peeled potato, boil for 30min, filter to take filtrate, and make up to 1.0l.

2. Atmosphere: aerobic

3, Temperature: 25-28 ℃

4. Notes:
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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