Gray tree flower|336080 |BNCC

Essential Information Certificate Related Products

Grifola frondosa(Fr.)S.F.Gray

Subculture procedure	① Dissolve the cryovial in a water bath (mold at 30 ℃) and shake rapidly to dissolve; ② Wipe the outer wall of the cryovial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates; ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Growth conditions	28 ℃; 7-10 days; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	Spread growth, filamentous, irregular edges, opaque, white front, flat shape, rough surface, wrinkled surface, dry texture, purity: pure
Sharing mode	Public welfare sharing

1.Description

1, Name: Grifolafrondosa(Fr.)S.F.Gray

2. BNCC No.: 336080

3. Biosafety level: 4

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1. Comprehensive PDA:20% potato juice 1L, glucose 20g ,KH2PO4 3g, MgSO4.7H2O 1.5g, thiamine trace, agar 15g,pH natural, 121 ℃,15min.

2. Atmosphere: aerobic

3, Temperature: 26 ℃,7-10 days

4. Notes:
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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