Aspergillus flavus|336156 |BNCC

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Subculture procedure	① Prepare 1-2 plates as mentioned above; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each Agar plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	28 ℃; 5-7 days; aerobic
Storage conditions	2-8 ℃
Safety level	2
morphology	Small filamentous fungi, with obvious colonies on a comprehensive PDA medium. The mycelium of the agar layer is white in the early stage, spreading and growing, and produces yellow spores in the later stage. The back of the medium is light yellow with folds
Sharing mode	Public welfare sharing

Aspergillus flavus Aspergillus

Storage conditions: 2~8 ℃

No.: 336156

Product format: freeze dried, 200ul

Validity period: 6 years

Biosafety level: 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Growth conditions:28 ℃, aerobic, PDA,5-7 days, PDA: potato boiling solution 1.0L, glucose 20.0g, agar 15.0g (liquid medium does not contain), natural pH. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.

Recovery steps:
(1)Prepare 1-2 of above mentioned plates;
(2)Open the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3)Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;
(4)Put the plates under the above culture conditions for cultivation for 5-7 days.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability:	good viability, in 5-7 days strain layer is obvious
colony morphology: (above)	Small filamentous fungi have obvious colonies on PDA medium, with white hyphae at the initial stage, dense and vigorous, and yellow spores growing on plates later.
Conclusion:	good viability, no abnormal colony morphology, qualified

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