Orange Penicillium|336236 |BNCC

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Penicillium citrnum Thom

Subculture procedure	① Prepare 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and pour it into the freeze-drying tube. After fully dissolving, mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all the plates under the above cultivation conditions, and once the strains grow, they can be used.
Growth conditions	28 ℃; 5-7 days; aerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	Spread and grow on comprehensive potato agar medium, small filamentous fungi, with white hyphae in the early stage and gray green spores in the later stage, yellow on the reverse side, wrinkled on the surface, hard texture, purity: pure
Sharing mode	Public welfare sharing

1.Description

1. Name:Penicillium citrnum Thom

2. BNCC No.:336236

3. Biosafety level: 4

2.Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1,Czapek′s Agar; S Agar: 30g sucrose, NaNO3 3g, MgSO4.7H2O 0.5g ,KCl 0.5g ,FeSO4.7H2O 0.01g, K2HPO4 1g, 13g agar, distilled water) 1000ml ,pH 7.2

2. Atmosphere: aerobic

3.Temperature: 25-26 ℃

4.Notes:

1.Normal culturing time, 1-2days for bacterial, 3days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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