Fusarium|338537 |BNCC

BeNa Culture Collection

Fusarium-BNCC
Fusarium-BNCC
  • BNCC
  • Fusarium sp.-BNCC
  • Fusarium sp.-BNCC

Fusarium sp.

Literatures(1)
  • Price: Contact
  • number:338537
  • Form:
    Small filamentous fungi grow well on PDA medium, with a pink front and spreading growth, and a yellow back
Standard strain Quantitative strain DNA extraction
Package:
Essential Information References Certificate Related Products
Fusarium sp.
Subculture procedure ① Dissolve the frozen tube in a 30 ℃ water bath and shake quickly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates; ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Growth conditions 28 ℃; 3-5 days; aerobic
Storage conditions -80 ℃
Safety level 1
morphology Small filamentous fungi grow well on PDA medium, with a pink front and spreading growth, and a yellow back
Sharing mode Public welfare sharing

Fusarium sp.

Storage conditions : 2~8 ℃

No. : 338537

Product format: agar slant in 14mm test tube 

Validity period : growing culture, in 30 dyas

Biosafety level : 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The agar slant can be used directly. In principle, it can be used many times without contamination within the validity period, but viability will gradually decrease with time. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :28 ℃, aerobic, PDA,3-5 days. PDA: potato boiling solution 1.0L, glucose 20.0g, agar 15.0g (not included in liquid medium), natural pH. Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L.

Recovery steps:
(1) Prepare 1-2 pieces of PDA plates; 
(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet; 
(3) Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2; 
(4) Lay flat the small pieces to the center of the agar plate;
(5) Put the plates under the above culture conditions, and the strains can be used when they grow.
Recovery record:  According to the recovery instructions, the results of the recovery are reported as follows:

Item test results
viability: good viability, in 4 days strain layer is obvious
colony morphology: (above)

small filamentous fungi with obvious colonies on PDA medium,

The hyphae are white, dense and vigorous, and the back of the culture medium is light yellow

Conclusion: good viability, no abnormal colony morphology, qualified
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