Du's anthracnose fungus|357947 |BNCC

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Colletotrichum dematium (Persoon : Fries) Grove

Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Growth conditions	28 ℃; 5-10 days; aerobic;
Storage conditions	-80 ℃
Safety level	1
morphology	Small filamentous fungi have obvious colonies on PDA medium, with white initial hyphae and raised colonies, and gray white later hyphae,
Sharing mode	Public welfare sharing

Colletotrichum dematium

Storage conditions : 2~8 ℃

No. : 357947

Product format :agar slant in 14mm test tube

Validity period: growing culture, in 30 days

Biosafety level : 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The agar slant can be used directly. In principle, it can be used many times without contamination within the validity period, but viability will gradually decrease with time. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :28°C, aerobic, integrated PDA, 5-10 days. Comprehensive PDA: Potato cooking liquid 1.0L, glucose 20.0g, KH2PO4 3.0g, MgSO4·7H2O 1.5g, trace amount of vitamin B1, agar 20.0g, pH 6.0±0.2. Sterilize at 121℃ for 15min. Potato cooking liquid: Weigh 200g of peeled potato pieces, boil in boiling water for 30min, collect the filtrate and dilute to 1.0L.

Recovery steps:

(1) Prepare 1-2 pieces of PDA plates;

(2) Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3) Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm²;

(4) Lay flat the small pieces to the center of the agar plate;

(5) Put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability， in 5-10days strain layer is obvious
colony morphology (above)	small filamentous fungi with obvious colonies on comprehensive PDA medium, The initial hyphae are white, the colonies are raised, the posterior hyphae are gray-white, and the back of the culture medium is light yellow
Conclusion	good viability , no abnormal colony morphology, qualified

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