Fusarium oxysporum|358442 |BNCC

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Fusarium oxysporum

Subculture procedure	① Dissolve the Frozen vial in a water bath (fungi at 30 ℃) and shake rapidly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates; ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Growth conditions	28 ℃; 5-7 days; aerobic;
Storage conditions	Liquid nitrogen
Safety level	1
morphology	Small filamentous fungi grow well on comprehensive PDA medium, with a white front and spreading growth, and a yellow back
Sharing mode	Public welfare sharing

Fusarium oxysporum

Storage conditions : 2~8 ℃

No. : 358442

Product format :agar slant in 14mm test tube

Validity : growing culture, in 30 days

Biosafety level : 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The agar slant can be used directly. In principle, it can be used many times without contamination within the validity period, but viability will gradually decrease with time. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28 ℃, aerobic, integrated PDA,5-7 days. Comprehensive PDA: potato boiling solution 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4·7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2 Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L.

Recovery steps:

① Prepare 1-2 integrated PDA tablets;

② After disinfection of the inclined surface of the test tube, open it in the safety cabinet;

③ Cut the pieces with sterile inoculation hoe and inoculation shovel (see attached page),0.5×0.5cm2 small square block;

④ Place the small square block flat on the center of the plate and on the agar surface;

⑤ The plate is being cultured under the above culture conditions, and the strains can be used when they grow out.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability in 5days,strain layer is obvious
colony morphology:	small filamentous fungi, the colony is obvious on the comprehensive PDA medium, white, dense and vigorous hyphae, spreading to the edge of the plate, and the back of the medium is light yellow.
conclusion: good viability, no abnormal colony morphology, qualified & nbsp;

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