Aspergillus oryzae|359118 |BNCC

Essential Information Certificate Related Products

Conidiobolus megalotocus Drechsler

Subculture procedure	① Dissolve the frozen tube in a water bath (fungi at 30 ℃) and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the cap of the tube, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (approximately 200 μ L per Agar plate); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Growth conditions	28 ℃; 7-10 days; aerobic;
Storage conditions	-80 ℃
Safety level	1
morphology	Spread growth, rough surface, white front, yellow back, light color
Sharing mode	Public welfare sharing

Conidiobolus megalotocus Drechsler

Storage conditions: 2~8 ℃

No. 359118

Product format: agar slant in 14mm test tube

Validity: growing culture, in 30 days

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receiving goods, please contact customer service within 24 hours in time. if it is overdue, it will be regarded as good receiving goods. The inclined plane of the test tube can be used directly. in principle, it can be used many times without pollution during the storage period, but its vitality will gradually decrease with time. Please operate in strict accordance with this instruction, otherwise the replacement service will not be provided if the strain is abnormal and inactivated.

Growth conditions: 28 ℃, aerobic, integrated PDA,7-10 days. Comprehensive PDA: potato boiling solution 1.0L, glucose 20.0g,KH₂PO₄ 3.0g,MgSO4·7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L.

Recovery steps:

(1) Prepare 1-2 pieces of PDA plates;

(2) sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3) cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm²;

(4) lay flat the small pieces to the center of the agar plate;

(5) put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability，in 7days strain layer is obvious
colony morphology: (above)	Small filamentous fungi have obvious colonies, white, dense hyphae and low flat on the integrated PDA medium, spreading and growing to the edge of the plate, and the back of the medium is light yellow.
Conclusion	good viability, no abnormal colony morphology, qualified

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