Hidden Mycobacterium|361313 |BNCC

BeNa Culture Collection

Hidden Mycobacterium-BNCC
  • BNCC
  • Mycobacterium celatum-BNCC

Mycobacterium celatum

  • Price: $ 223
  • number:BNCC361313
  • MPs:
    The colony is small, round, with irregular edges, opaque, yellow on the front, light in color, raised in the middle, dry in texture, G+(blue purple), rod-shaped
Essential Information Related Products
Mycobacterium celatum
Culture medium BNCC360851
Description Middlebrook 7H10 Agar
Composition (NH4)2SO4:0.5g, KH2PO4:1.5g, Na2HPO4:1.5g, Na3C6H5O7:0.4g, MgSO4:0.025g, CaCl2:0.5mg, ZnSO4:0.001g, CuSO4:0.001g, Sodium L-Glutamate(C5H8NNaO4):0.5g, C6H8FeNO7:0.04g, Pyridoxine Hydrochloride(C8H11NO3.HCl):0.001g, Biotin(C10H16N2O3S):0.5mg, Malachite Green:0.25mg, OADC Supplement:10%(added at 50℃), Glycerol:5ml, Agar:15.0g, pH:6.4-6.8(25℃)
Growth conditions 37 ℃; 7-14 days; aerobic
Subculture procedure ① Prepare 2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. (During the cultivation period, the agar plate should be kept moist)
Storage conditions 2-8 ℃
Safety level 2
morphology The colony is small, round, with irregular edges, opaque, yellow on the front, light in color, raised in the middle, dry in texture, G+(blue purple), rod-shaped
Sharing mode Public welfare sharing
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