Clostridium difficile|364175 |BNCC

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Clostridium difficile-BNCC
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  • Clostridioides difficile-BNCC

Clostridioides difficile

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  • number：364175
  • Form：
    The colony diameter is 0.5-1mm, circular, with irregular edges, opaque, gray white on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G+(blue purple), Bacillus, purity: pure
Standard strain Quantitative strain DNA extraction
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Clostridioides difficile
Subculture procedure ① Dissolve the frozen tube in a 37 ℃ water bath and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to the anaerobic workstation for operation; ③ Unscrew the tube cap, extract all the dissolved solution, and transfer it into 1-2 agar plates (for anaerobic bacteria cultivation, the culture medium should be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After uniform coating, transfer to the above cultivation conditions for cultivation. ⑤ The anaerobic cultivation environment can also be carried out in a 350ml anaerobic cultivation bag containing anaerobic gas generator.
Growth conditions 37 ℃; 24-48 hours; anaerobic
Storage conditions -80 ℃
Safety level 2
morphology The colony diameter is 0.5-1mm, circular, with irregular edges, opaque, gray white on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G+(blue purple), Bacillus, purity: pure
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