Chelated Mycobacterium|364252 |BNCC

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Mycobacterium chelonae

Culture medium	BNCC360851
Description	Middlebrook 7H10 Agar
Composition	(NH₄)₂SO₄:0.5g, KH₂PO₄:1.5g, Na₂HPO₄:1.5g, Na₃C₆H₅O₇:0.4g, MgSO₄:0.025g, CaCl₂:0.5mg, ZnSO₄:0.001g, CuSO₄:0.001g, Sodium L-Glutamate(C₅H₈NNaO₄):0.5g, C₆H₈FeNO₇:0.04g, Pyridoxine Hydrochloride(C₈H₁₁NO₃.HCl):0.001g, Biotin(C₁₀H₁₆N₂O₃S):0.5mg, Malachite Green:0.25mg, OADC Supplement:10%(added at 50℃), Glycerol:5ml, Agar:15.0g, pH:6.4-6.8(25℃)
Growth conditions	28 ℃; 5-7 days; aerobic;
Subculture procedure	① Prepare 1 sterile water and 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and pour it into the freeze-drying tube to dissolve and mix thoroughly; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows.
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, rough and wrinkled on the surface, dry texture, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing