BeNa Culture Collection
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| Subculture procedure | ① Prepare 1-2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Growth conditions | 37 ℃; 4-5 days; 5%CO2； |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony has a diameter of 0.5-1mm, irregular shape, irregular edges, yellow brown on the front, dark and rough on the surface, dry texture, G+(blue purple), Bacillus subtilis, purity: pure |
| Sharing mode | Public welfare sharing |
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