BeNa Culture Collection
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| Subculture procedure | ① Prepare the above 2 plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. (During the cultivation period, the agar plate should be kept moist) |
| Growth conditions | 37 ℃; 10-15 days; aerobic; |
| Storage conditions | 2-8 ℃ |
| Safety level | 2 |
| morphology | The colony is gray white, dry and difficult to pick up, with bacteria and positive acid resistant staining |
| Sharing mode | Public welfare sharing |