BeNa Culture Collection
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| Culture medium | BNCC362337 |
| Description | Chocolate Agar Plate |
| Composition | Polypeptone:18.0g, Starch:1.0g, NaCl:5.0g, Agar:15.0g, Defibrinated Sheep Blood:40mL, pH:7.4±0.2(25℃) |
| Growth conditions | 37 ℃; 5-7 days; 5%CO2; |
| Subculture procedure | ① Prepare 2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 2 |
| morphology | Colonies with a diameter greater than 2mm, irregular shape, irregular edges, opaque, yellow front, flat shape, slightly raised, gray surface, rough surface, dry texture, G+(blue purple), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
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