Bacillus subtilis|189983 |BNCC

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Bacillus subtilis

Subculture procedure	① Prepare 2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Growth conditions	37 ℃; 18-24h; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 2-4mm, circular, with irregular edges, opaque, dark yellow on the front, flat in shape, rough on the surface, dry in texture, G+(blue purple), rod-shaped, purity: pure
Sharing mode	Public welfare sharing

Bacillus subtilis

BNCC No.: 189983

Biosafety level: 4

Storage condition：

Storage of freezed dried ampoule and agar slant at 2°C to 8°C.

Growth Conditions:

1, Nutrition gravy medium : peptone 5.0g, beef extract 3.0g,NaCl 5.0g, agar 15.0g, distilled water 1.0L,pH7.0. [note] adding 5mg MnSO₄·H₂O when culturing Bacillus,which is good for producing spores.

2. Atmosphere: aerobic

3. Temperature: 37 ℃

Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.

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