Botrytis cinerea|117822 |BNCC

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Inonotus obliquus (Ach. ex Pers.) Pilát

Subculture procedure	① Prepare one 90mm solid agar plate, one inoculation shovel and one inoculation hoe; ② Inoculation shovel, inoculation hoe, flame burn, cool for later use; ③ After disinfecting the outer wall of the inclined surface, open it in a sterile environment, first use an inoculation hoe, and then use an inoculation shovel to cut into pieces; ④ Inoculate the bacterial block with a spatula, place it on the surface of a agar plate, and cover the agar plate; ⑤ Place the flat agar plate in the fungi incubator and cultivate it according to the above conditions. Do not seal it with a sealing film. The agar plate can be used when it is almost full.
Growth conditions	28 ℃; 7-9 days; aerobic
Storage conditions	2-8 ℃
Safety level	1
morphology	Spreading growth, white to yellowish-brown on the obverse, black on the reverse, with a rough and dull surface and dry texture.
Sharing mode	Public welfare sharing

Inonotus obliquus

Storage conditions : 2~8 ℃

No. : 117822

Product format: agar slant in 14mm test tube

Validity period: 30 days

Biosafety level : 1, handle in ultra-clear table or safety cabinet

Receiving notice:if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :25-28 ℃, aerobic, integrated PDA. Comprehensive PDA:20% potato juice 1L, glucose 20g ,KH₂PO₄ 3g, MgSO₄.7H₂O 1.5g, thiamine trace, agar 15g,pH natural.

Recovery steps:

(1)Prepare 1-2 pieces of PDA plates;

(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3)Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page),the size of square pieces is 0.5 × 0.5cm2;

(4)Lay flat the small pieces to the center of the agar plate;

(5)Put the plates under the above culture conditions for 5-7 days. You can also contact customer service for information "Inclined Transfer Method-Filamentous Fungi".

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability, in 7 days plate colony is obvious
colony morphology:	small filamentous fungi, colonies are obviously visible on comprehensive PDA culture medium, hyphae become yellowish brown with ring patterns after white, colonies edge hyphae are fine.
Conclusion:	good viability, no abnormal colony morphology, completely consistent with the above figure, qualified

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