Radiation resistant Acinetobacter baumannii|271748 |BNCC

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Acinetobacter radioresistens

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	30 ℃, 18-24h, aerobic
Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid medium and two agar plates.② Within a biological safety cabinet, surface-sterilize and open the glass ampoule. Briefly flame the neck, then immediately apply a drop of sterile water to induce thermal cracking. Carefully open it using sterile forceps.③ Aspirate 0.5 mL of the liquid medium and inject it into the freeze-dried vial. Mix thoroughly to achieve complete resuspension. Transfer the entire suspension back into the original liquid medium test tube and mix well.④ Inoculate 0.2 mL of the resulting bacterial suspension onto each agar plate and spread evenly. Repeat this step to prepare a duplicate Agar plate.⑤ Incubate both the liquid culture tube and all agar plates under the aforementioned culture conditions. The culture is ready for use once visible growth is observed.
Storage conditions	2-8 ℃
Safety level	2
morphology	Colonies are 1-2 mm in diameter, circular with entire margins, and opaque. They are yellow, convex, and exhibit a smooth, shiny surface with a moist and viscous texture.
Sharing mode	Public welfare sharing