Radiation resistant Acinetobacter baumannii|271748 |BNCC

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Acinetobacter radioresistens

Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid medium and two agar plates.② Within a biological safety cabinet, surface-sterilize and open the glass ampoule. Briefly flame the neck, then immediately apply a drop of sterile water to induce thermal cracking. Carefully open it using sterile forceps.③ Aspirate 0.5 mL of the liquid medium and inject it into the freeze-dried vial. Mix thoroughly to achieve complete resuspension. Transfer the entire suspension back into the original liquid medium test tube and mix well.④ Inoculate 0.2 mL of the resulting bacterial suspension onto each agar plate and spread evenly. Repeat this step to prepare a duplicate Agar plate.⑤ Incubate both the liquid culture tube and all agar plates under the aforementioned culture conditions. The culture is ready for use once visible growth is observed.
Growth conditions	30 ℃, 18-24h, aerobic
Storage conditions	2-8 ℃
Safety level	2
morphology	Colonies are 1-2 mm in diameter, circular with entire margins, and opaque. They are yellow, convex, and exhibit a smooth, shiny surface with a moist and viscous texture.
Sharing mode	Public welfare sharing

Edwardsiella tarda

Storage conditions : 2~8 ℃

No. : 271748

Product format: freeze dried, 200ul

Validity : 6 years

Biosafety level : 2 , handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :30 ℃, aerobic, nutrient agar medium, 18-24h. Nutritional agar medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH7.0. Sterilization at 121 ℃ for 15min.

Recovery steps:
(1 )Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;
(2) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;
(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;
(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 18-24h liquid medium become turbid, obvious strain layer occurs on the plate,colony is typical on marked plate.
colony morphology: (above)	Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque color: yellow uplift: middle convex surface: bright and smooth texture: moist and viscous
Conclusion:	good viability, no abnormal colony morphology, qualified

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