Genus Trichoderma|112764 |BNCC

Essential Information Certificate Related Products

Pestalotiopsis sp.

Subculture procedure	① Prepare one 90mm solid plate, one inoculation shovel and one inoculation hoe; ② Inoculation shovel, inoculation hoe, flame burn, cool for later use; ③ After disinfecting the outer wall of the inclined surface, open it in a sterile environment, first use an inoculation hoe, and then use an inoculation shovel to cut into pieces; ④ Inoculate the bacterial block with a spatula, place it on the surface of a agar plate, and cover the plate; ⑤ Place the flat plate in the mold incubator and cultivate it according to the above conditions. Do not seal it with a sealing film. The plate can be used when it is almost full.
Growth conditions	28 ℃; 5-7 days; Aerobic
Storage conditions	-80 ℃
Safety level	1
morphology	Small filamentous fungi have obvious colonies on comprehensive PDA medium, with white hyphae in the early stage, dense and vigorous hyphae, spreading and growing towards the edge of the plate, and a light pink fungal layer in the later stage
Sharing mode	Public welfare sharing

Pestalotia sp.

Storage conditions: 2~8 ℃

No. 112764

Product format: agar slant in 14mm test tube

Validity period: growing culture, in 30 days

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 28°C, aerobic, Integrated PDA, 5-7. Comprehensive PDA: Potato cooking liquid 1.0L, glucose 20.0g, KH2PO4 3.0g, MgSO4 7H2O 1.5g, trace amount of vitamin B1, agar 20.0g, pH 6.0±0.2. Sterilize at 121℃ for 15min. Potato cooking liquid: Weigh 200g of peeled potato pieces, boil in boiling water for 30min, collect the filtrate and dilute to 1.0L.

Recovery steps:

(1)Prepare 1-2 pieces of PDA plates;

(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3)Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm²;

(4)Lay flat the small pieces to the center of the agar plate;

(5)Put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability, in 5-7 days plate colony is obvious
colony morphology:	small filamentous fungi with obvious colonies on comprehensive PDA medium and white initial hyphae, The hyphae are dense and vigorous, spreading to the edge of the plate, and the layer is light red in the later stage.
Conclusion:	good viability, no abnormal colony morphology, qualified

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