Cocoa hair color fungus|119691 |BNCC

Essential Information Certificate Related Products

Lasiodiplodia theobromae (Pat.) Griffon & Maubl.

Subculture procedure	① Dissolve the Frozen vial in a water bath (fungi at 30 ℃) and shake rapidly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (each Agar plate contains approximately 200 μ L, and for anaerobic bacteria cultivation, the culture medium needs to be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Growth conditions	28 ℃; 3-5 days; aerobic
Storage conditions	-80 ℃
Safety level	1
morphology	Spread and grow on PDA medium, with white fuzzy mycelium in the early stage and dark olive color in the later stage. The mycelium is dense and developed, and the back of the medium is black
Sharing mode	Public welfare sharing

Lasiodiplodia theobromae

Storage conditions : 2~8 ℃

No.: 119691

Product format : agar slant in 14mm test tube

Validity period: 30 days

Biosafety level : 1, handle in ultra-clear table or safety cabinet

Receiving notice:if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:25-28 ℃, aerobic, integrated PDA. Comprehensive PDA:20% potato juice 1L, glucose 20g ,KH₂PO₄ 3g, MgSO₄.7H₂O 1.5g, thiamine trace, agar 15g,pH natural.

Recovery steps:

(1)Prepare 1-2 pieces of PDA plates;

(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

(3)Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2;

(4)Lay flat the small pieces to the center of the agar plate;

(5)Put the plates under the above culture conditions for 5-7 days. You can also contact customer service for information "Inclined Transfer Method-Filamentous Fungi".

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability,in 6 days plate colony is obvious
colony morphology:	small filamentous fungi, the colony is obviously visible on the comprehensive PDA medium, the colony is gray-white at the beginning, then turns gray-brown to brown-black, flocculent;shape, spread quickly.
Conclusion:	good viability, no abnormal colony morphology, completely consistent with the above figure, qualified

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