Fake Mycobacterium tuberculosis|368433 |BNCC

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Corynebacterium pseudotuberculosis

Culture medium	BNCC360798
Description	Colombia Agar Chocolate Plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:20.0g, Defibrinated Sheep Blood:5%, pH:7.3±0.2(25℃)
Growth conditions	37 ℃; 48-72h；5%CO2；
Subculture procedure	Prepare 1-2 fresh agar plates (placed in an anaerobic environment for 24 hours of deoxygenation) with typical bacterial colonies; ② Open the safety cabinet, burn the top with an alcohol lamp, then quickly drip sterile water (placed in an anaerobic environment for 24 hours to remove oxygen) to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows.
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony diameter is 1-2mm, circular, with irregular edges, opaque, gray white front, smooth surface, moist texture, G+(blue purple), Bacillus subtilis, purity: pure
Sharing mode	Public welfare sharing