Gas producing Collinsella bacteria|371782 |BNCC

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Collinsella aerofaciens

Culture medium	BNCC363892
Description	Modified PYG Broth
Composition	Pancreatic Digest of Casein:5.0g, Peptone:5.0g, Yeast Extract:10.0g, Beef Extract:5.0g, Glucose:5.0g, K₂HPO₄:2.0g, Tween 80:1.0ml, CaCl₂.2H₂O:0.01g, MgSO₄.7H₂O:0.02g, K₂HPO₄:0.04g, KH₂PO₄:0.04g, NaHCO₃:0.4g, NaCl:0.08g, Resazurin:1.0mg, Hemin:5mg, Vitamin K1:1mg, C₃H₇NO₂S.HCl:0.5g, pH:7.2±0.2(25℃)
Growth conditions	37 ℃; 48-72 hours; anaerobic
Subculture procedure	① Prepare one 18mm test tube of liquid culture medium, approximately 10mL (placed in anaerobic environment for 24 hours of deoxygenation in advance); ② Disinfect the surface of the ampoule tube, open it in a safe cabinet, burn the top with an alcohol lamp, quickly add sterile water to the burning area to break it, and then use tweezers to knock it open; ③ Transfer approximately 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back to the liquid culture medium in the test tube; ④ Place the liquid test tube under the specified "culture conditions" mentioned above for cultivation. If the bacterial liquid is turbid or there is a large amount of bacterial growth at the bottom, it can be used.
Storage conditions	2-8 ℃
Safety level	2
morphology	G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing